Induction of Tolerance in Immunologically Competent Primates

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Abstract
Immunologic tolerance has been induced in mice, rats, guinea pigs, rabbits and chickens with protein and polysaccharide antigens (1, 2). In addition, tolerance has been produced in fetal rhesus monkeys with large doses of bovine γ globulin and in newborn humans with blood group antigens (3, 4). However, the induction of immunologic tolerance has not been reported in older primates. We report here the induction of tolerance to human γ globulin (HGG) in immunologically competent monkeys. Six-month- and one-year-old rhesus monkeys (Macaca mulatta) were bred and maintained at Bionetric Research Laboratories, Inc., Kensington, Maryland. In the first experiment, 6-month-old monkeys weighing 1500 to 2000 g were used. Animals were treated in a manner similar to that used to induce tolerance in adult mice (5, 6). Ten milligrams of HGG/ml (Cohn Fraction II, Hyland Laboratories, Costa Mesa, Calif.) in pyrogen-free saline was centrifuged for 2 hr at 105,000 × G in a Spinco model L-2 ultracentrifuge to remove aggregated proteins.

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