Polysome transitions and the regulation of ribonucleic acid synthesis in Escherichia coli.
- 1 July 1966
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 56 (1) , 262-268
- https://doi.org/10.1073/pnas.56.1.262
Abstract
In a stringent strain of Eseherichia coli, the polysomes disappeared when net RNA synthesis was inhibited by amino acid starvation. Polysomes appeared under conditions where net RNA synthesis resumed, both in the presence and absence of protein synthesis. Polysomes did not disappear in a relaxed strain, where RNA synthesis continues during amono acid starvation. Thus, there is a direct correlation between the level of polysomes and the synthesis of net RNA. Since mRNA synthesis apparently continues during amino acid starvation, the disappearance of polysomes must result from the failure of the ribosomes to re-attach to mRNA. The mechanism is discussed whereby the dissociation of polysomes could result in an inhibition of stable RNA formation and prevent the incorporation of exogenous precursors into mRNA.This publication has 24 references indexed in Scilit:
- The inhibition by chloramphenicol of nascent protein formation in E. coli.Proceedings of the National Academy of Sciences, 1966
- Synthesis and breakdown of messenger RNA without protein synthesisJournal of Molecular Biology, 1966
- The stimulation of RNA synthesis by ribosomes invitroBiochemical and Biophysical Research Communications, 1966
- INDUCED ENZYME FORMED ON BACTERIAL POLYRIBOSOMESProceedings of the National Academy of Sciences, 1964
- The Regulation of RNA Synthesis in BacteriaProgress in Nucleic Acid Research and Molecular Biology, 1964
- Inhibition of RNA polymerase by RNAJournal of Molecular Biology, 1963
- Kinetic of induced enzyme synthesis determination of the mean life of galactosidase-specific messenger RNABiochimica et Biophysica Acta, 1963
- Regulation of ribosomal and transfer RNA synthesisJournal of Molecular Biology, 1962
- A GENETIC LOCUS FOR THE REGULATION OF RIBONUCLEIC ACID SYNTHESISProceedings of the National Academy of Sciences, 1961
- Turnover of protein in growing and non-growing populations of Escherichia coliBiochemical Journal, 1958