Quantitative measurement of prostaglandins E2 and E3 by selected ion monitoring

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Abstract
A method for the simultaneous quantative analysis of prostaglandin E2 (PGE2) and PGE3 is described. The PG were analyzed by selected ion monitoring as the methyl ester‐TMS ether derivatives of PGB2 and PGB3, respectively. The internal standard for the quantification of both species was [3,3,4,4‐2H4]PGE2. A linear response over the range 0.6–50 ng (1.7–143 pmoles) was demonstrated for PGE3. The chromatographic conditions used (2% SP‐2330 column) afforded nearly baseline separation of the prostaglandins. New standard curves for PGE3 must be developed each time the ion source parameters are changed. In a typical calibration run, the instrumental precision, expressed as coefficient of variation, ranged from 1.1 to 7.2% for PGE2 (3 to 100 ng injected) and from 1.6 to 11.1% for PGE3 (1.5–50 ng injected). The method was applied to the PG analysis of rat renomedullary tissues. The recovery of synthetic PGE2 added to medullary homogenates was 100.5±1.7% (mean±SEM, n=9), and the recovery of PGE3 was 91.3±1.4% (n=9).