Effects of Cortisone on Susceptibility of Mice toListeria Monocytogenes

Publisher Website
Google Scholar
Abstract
Subcutaneous administration of 12 to 16 mg of hydrocortisone acetate to white Swiss mice weighing 18 to 20 g reduced the LD50 of Listeria monocytogenes from 104 or 105 to less than 50 bacteria. When listeriosis is suspected but cannot be confirmed by means of conventional culture methods, isolation of L. monocytogenes from tissues and exudates may be accomplished by injection of 5 or 6 mice treated with cortisone. The mice should be observed for 7 days. Exudates in 0.5-ml amounts can be inoculated directly into the peritoneal cavity of the mouse. Tissue can be prepared for inoculation by grinding 1 g of tissue in 10 ml of broth with sterile alundum. The gross particles are separated by centrifugation, and 0.5 ml of the supernatant inoculated intraperitoneally.

This publication has 3 references indexed in Scilit: