SPECIFIC IDENTIFICATION OF INTRACELLULAR IMMUNOGLOBULIN IN PARAFFIN SECTIONS OF MULTIPLE-MYELOMA AND MACROGLOBULINEMIA USING AN IMMUNOPEROXIDASE TECHNIQUE

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  • 1 January 1977
    • journal article
    • research article
    • Vol. 87  (1) , 47-57
Abstract
Paraffin-embedded tissues of 47 [human] cases of multiple myeloma and 4 cases of macroglobulinemia were studied by the immunoperoxidase indirect sandwich technique for identification of intracytoplasmic immunoglobulin [Ig]. Specific Ig with a single H and/or L chain was readily detected in the neoplastic cells. Studies were performed on iliac crest bone marrow biopsies which were fixed and decalcified using Zenker''s-acetic acid solution and on tissues from various sites which were fixed in formalin. An excellent correlation was obtained between the specific Ig detected intracellularly in the plasma cells or lymphoplasmacytic cells and the monoclonal Ig found in serum or urine of these cases. The immunoperoxidase technique represents a sensitive and specific method for identifying intracellular Ig in paraffin-embedded tissues.