Diurnal Variations and within the Feed in Lactose and Oligosaccharides of Human Milk

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Abstract
Variations of lactose and oligosaccharides in human milk were studied during the day and the feed, by dialysis, chromatography of Bio-gel P-2 column, paper chromatography, colorimetric analysis and gas chromatography. Progressive changes were found during the day, but not during the feed. A diurnal variation in milk lactose was seen, which was the inverse of the oligosaccharides. Significant negative correlations between lactose and oligosaccharides were seen in the afternoon and evening (p < 0.05). The minimal mean value of lactose at 19.00 h is 58.64 .+-. 5.28 g/l and the maximal mean values of oligosaccharides was 17.93 .+-. 2.28 g/l. The data are discussed with regard to other milk constituents and to their possible physiological relevance.

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