Lipase and its action

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Abstract
Lipase acted at the phase interface in the system lipase-glycerol extract-oleic acid. The lipase disappeared from the glycerol and accumulated at the phase interface. The accumulation took place in 2 ways: the 1st was of the nature of ad-sorption, and the 2nd consisted of the formation of a membrane at the phase interface. Only the lipase present at the phase interface was active. The reaction of synthesis of olein seemed to be bimolecular in nature. The initial velocity of reaction was proportional to the wt. of aqueous phase taken. The reaction was accelerated by increasing the concentration of glycerol; i.e., the equilibrium point was shifted in the direction of synthesis. The initial velocity of reaction was directly proportional to the relative concentration of enzyme present.

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