Separation of enantiomers of N‐acetylcysteine by capillary electrophoresis after derivatization by o‐phthaldialdehyde
- 1 January 1994
- journal article
- Published by Wiley in Electrophoresis
- Vol. 15 (1) , 763-768
- https://doi.org/10.1002/elps.11501501106
Abstract
The optical purity of N‐acetyl‐L‐cysteine was tested by capillary electrophoresis. The D‐enantiomer can be found down to a ratio of 0.4:99.6 within 4 min without the use of the D‐form or the racemate as a standard. The efficiency was optimized during method development up to a theoretical plate number, N, of several hundred thousand. The migration order and the separation mechanism was explained. The assumed structure of the formed isoindole S‐[2‐(1‐carboxy‐2‐methylpropyl)isoindole‐1‐yl]‐N‐acetylcysteine diastereomers was confirmed and its stability was examined.Keywords
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