Immunologic Tolerance: Role of the Regional Lymph Node

Abstract
Dansyl chloride, a skin sensitizer when injected in complete Freund's adjuvant, induced marked tolerance and no sensitization when applied to the intact skin of guinea pigs. Application of this chemical to alymphatic skin islands failed to induce tolerance or sensitization. Lymphatic connections between skin and regional lymph nodes were essential for the development of immunologic tolerance.