NECATOR-AMERICANUS AND ANCYLOSTOMA-CEYLANICUM - DEVELOPMENT OF PROTOCOLS FOR DUAL INFECTION IN HAMSTERS

  • 1 January 1985
    • journal article
    • research article
    • Vol. 42  (1) , 45-54
Abstract
Two-day-old baby hamsters were infected initially with the infective larvae of hamster-adapted human hookworm, N. americanus (NaL3). After a specified period they were again infected orally with infective larvae of A. ceylanicum (AcL3). Three weeks after the 2nd infection they were killed and the establishment of N. americanus and A. ceylanicum was assessed. The effect of different infection levels and exposure period of N. americanus on the concurrent establishment of A. ceylanicum was also examined. An infection with 50 NaL3 percutaneously, and 3 wk later, a 2nd infection with 50 AcL3 orally has produced resonably equal number of hookworms (no statistical difference in the burden of N. americanus and A. ceylanicum) inthe intestine of hamsters. This protocol of dual infection was suitable to develop 2 spp. of hookworms in hamsters for anthelmintic screening.