Band-Broadening Effects in a Column-Switching System for HPLC

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Abstract
Band-broadening contributions of the individual components of a column-switching system for HPLC have been experimentally determined and, where possible, compared with theoretically predicted values. The agreement was generally good. As the system was designed for the analysis of trace components in an environmental sample, special attention was paid to the implications of increasing sample volume in terms of the effect upon detection limits and errors in quantification. For the column system employed, a sample volume of 100 µl was shown to give the best compromise.

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