Comparison of Adsorption Coefficient (KOC) for Soils and HPLC Retention Factors of Aromatic Hydrocarbons Using a Chemically Immobilized Humic Acid Column in RP-HPLC

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Abstract
The determination of soil adsorption coefficients (KOC) via HPLC capacity factors (k′) has been studied, including the effect of column type and mobile phase composition on the correlation between log KOC and log k′. KOC values obtained by procedures other than HPLC correlate well with HPLC capacity factors determined on a chemically immobilized humic acid stationary phase, and it is suggested that this phase is a better model for the sorption onto soil or sediment than the octadecyl-, phenyl- and ethylsilica phases. By using log k′w a theoretical capacity factor has been obtained by extrapolation of the retention data in a binary solvent system to pure aqueous eluent. There is a better correlation between log KOC and log k′w than the correlation between log KOC and log k′.