A mitochondrial DNA sequence is associated with abnormal pollen development in cytoplasmic male sterile bean plants.
- 1 April 1992
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 4 (4) , 435-449
- https://doi.org/10.1105/tpc.4.4.435
Abstract
Cytoplasmic male sterility (CMS) in common bean is associated with the presence of a 3-kb unique mitochondrial sequence designated pvs. The pvs sequence encodes at least two open reading frames (297 and 720 bp in length) with portions derived from the chloroplast genome. Fertility restoration by the nuclear restorer gene Fr results in the loss of this transcriptionally active unique region. We examined the effect of CMS (pvs present) and fertility restoration by Fr (pvs absent) on the pattern of pollen development in bean. In the CMS line, pollen aborted in the tetrad stage late in microgametogenesis. Microspores maintained cytoplasmic connections throughout pollen development, indicating aberrant or incomplete cytokinesis. Pollen-specific events associated with pollen abortion and fertility restoration imply that a gametophytic factor or event may be involved in CMS. In situ hybridization experiments suggested that significant reduction or complete loss of the mitochondrial sterility-associated sequence occurred in fertile pollen of F2 populations segregating for fertility. These observations support a model of fertility restoration by the loss of a mitochondrial DNA sequence prior to or during microsporogenesis/gametogenesis.Keywords
This publication has 12 references indexed in Scilit:
- RNA-mediated transfer of the gene coxII from the mitochondrion to the nucleus during flowering plant evolutionCell, 1991
- Fertility Restoration Is Associated with Loss of a Portion of the Mitochondrial Genome in Cytoplasmic Male-Sterile Common Bean.Plant Cell, 1990
- The complete sequence of the rice (Oryza sativa) chloroplast genome: Intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cerealsMolecular Genetics and Genomics, 1989
- Mitochondrial DNA rearrangement associated with fertility restoration and cytoplasmic reversion to fertility in cytoplasmic male sterile Phaseolus vulgaris LProceedings of the National Academy of Sciences, 1988
- Mutation to male fertility and toxin insensitivity in Texas (T)-cytoplasm maize is associated with a frameshift in a mitochondrial open reading frameProceedings of the National Academy of Sciences, 1987
- Some New Methods for Affixing Sections to Glass Slides. I. Aqueous AdhesivesStain Technology, 1987
- Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the texas male-sterile cytoplasmCell, 1986
- The sequence of the gene for cytochromecoxidase subunit I, a frameshift containing gene for cytochromecoxidase subunit II and seven unassigned reading frames inTrypanosoma bruceimitochondrial maxi-cirle DNANucleic Acids Research, 1984
- Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probesVirology, 1983
- Determination of Nucleotide Sequences in DNAScience, 1981