Regulation of glycine tRNA gene expression in the posterior silk glands of the silkworm Bombyx mori.
- 1 February 1992
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 89 (3) , 1070-1074
- https://doi.org/10.1073/pnas.89.3.1070
Abstract
The glycine tRNA genes in silkworm Bombyx mori contain two regulatory regions upstream of the transcription start site as identified by direct transcription of 5' deletion mutants, transcription competition, gel mobility shift assays, and footprinting. A positive regulatory region is present in the immediate 5' flanking sequences of the four tRNA 1Gly clones studied. This region is essential for cell-free transcription in homologous extracts. A negative regulatory region is present farther upstream, and transcription competition experiments indicate its presence in three of the four clones examined.Keywords
This publication has 21 references indexed in Scilit:
- S. cerevisiae TFIIIB is the transcription initiation factor proper of RNA polymerase III, while TFIIIA and TFIIIC are assembly factorsCell, 1990
- Footprinting DNA-protein complexes in situ following gel retardation assays using 1,10-phenanthroline-copper ion: Escherichia coli RNA polymerase-lac promoter complexesBiochemistry, 1987
- A rapid and sensitive method for the estimation of individual tRNA poolsBiochemical and Biophysical Research Communications, 1986
- 5′-flanking sequences that inhibit in vitro transcription of a Xenopus laevis tRNA geneCell, 1983
- Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nucleiNucleic Acids Research, 1983
- Silkworm 5S RNA and Alanine tRNA Genes Share Highly Conserved 5′ Flanking and Coding SequencesMolecular and Cellular Biology, 1982
- Two control regions for eukaryotic tRNA gene transcription.Proceedings of the National Academy of Sciences, 1980
- The nucleotide sequence of two silk gland alanine tRNAs: Implications for fibroin synthesis and for initiator tRNA structureCell, 1977
- Functional adaptation of tRNA populationJournal of Theoretical Biology, 1974
- The Silk FibroinsAdvances in Protein Chemistry, 1958