DENSITY GRADIENT CENTRIFUGATION FOR SEPARATION OF DIFFERENT STAGES OF TOXOPLASMA-GONDII

  • 1 January 1979
    • journal article
    • research article
    • Vol. 26  (1) , 9-13
Abstract
Suitable procedures for separation of various stages of T. gondii by density gradient centrifugation were delineated using peritoneal exudate of infected mouse as a model. Separation from host cells was effected by gradient centrifugation at 450 g for 30 min. Using Ficoll, dextran and sucrose, average parasite recoverites by pooling up to and including the peak fraction of 73.65, 66.18 and 65.58%, respectively, were obtained. T. gondii trophozoites peaked at density of 1.040 g/ml with Ficoll, 1.060 g/ml with dextran and 1.110 g/ml with sucrose. In view of successful separation of exo-enteric stages of T. gondii by density gradient centrifugation, possible application of this method to isolation of various endo-enteric stages is discussed.

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