The cardiac troponin T alternative exon contains a novel purine-rich positive splicing element.
Open Access
- 1 June 1993
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 13 (6) , 3660-3674
- https://doi.org/10.1128/mcb.13.6.3660
Abstract
We have characterized a novel positive-acting splicing element within the developmentally regulated alternative exon (exon 5) of the cardiac troponin T (cTNT) gene. The exon splicing element (ESE) is internal to the exon portions of the splice sites and is required for splicing to the 3' splice site but not the 5' splice site flanking the exon. Sequence comparisons between cTNT exon 5 and other exons that contain regions required for splicing reveal a common purine-rich motif. Sequence within cTNT exon 5 or a synthetic purine-rich motif facilitates splicing of heterologous alternative and constitutive splice sites in vivo. Interestingly, the ESE is not required for the preferential inclusion of cTNT exon 5 observed in primary skeletal muscle cultures. Our results strongly suggest that the purine-rich ESE serves as a general splicing element that is recognized by the constitutive splicing machinery.Keywords
This publication has 62 references indexed in Scilit:
- Identification of exon sequences and an exon binding protein involved in alternative RNA splicing of calcitonin/CGRPNucleic Acids Research, 1992
- A role for exon sequences in alternative splicing of the human fibronection geneNucleic Acids Research, 1987
- High-efficiency transformation of mammalian cells by plasmid DNA.Molecular and Cellular Biology, 1987
- Signals for the selection of a splice site in pre-mRNAJournal of Molecular Biology, 1987
- The length but not the sequence of the polyoma virus late leader exon is important for both late RNA splicing and stabilityNucleic Acids Research, 1987
- The role of nucleotide sequences in splice site selection in eukaryotic pre-messenger RNANature, 1986
- A role for exon sequences and splice-site proximity in splice-site selectionCell, 1986
- Spontaneous splicing mutations at the dihydrofolate reductase locus in Chinese hamster ovary cells.Molecular and Cellular Biology, 1986
- The chicken fast skeletal troponin I gene: exon organization and sequenceNucleic Acids Research, 1986
- Exon mutations that affect the choice of splice sites used in processing the SV40 late transcriptsNucleic Acids Research, 1985