The Influence of Temperature on Growth, Sporulation, and Heat Resistance of Spores of Six Strains of Clostridium perfringens

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Abstract
The influence of temperature on growth, sporulation, and heat resistance of six strains of Clostridium perfringens was examined. The range of temperatures for growth and sporulation varied between strains and was influenced by the culture medium. Optimum growth occurred in Thioglycolate medium between 30 and 40 C for all strains. Optimum sporulation in Ellner's medium occurred in the 37 to 40 C range for all strains. The maximum number of spores produced varied with the strain. Heat resistance of the spores varied between strains but was not modified by temperature of incubation.