Use of a Monoclonal Antibody for the Therapeutic Monitoring of Cyclosporine in Plasma and Whole Blood
- 1 December 1988
- journal article
- research article
- Published by Wolters Kluwer Health in Therapeutic Drug Monitoring
- Vol. 10 (4) , 453-458
- https://doi.org/10.1097/00007691-198804000-00013
Abstract
The evaluation of a radioimmunoassay (RIA) for the monitoring of cyclosporine (CSA) that utilizes a monoclonal antibody specific for the parent compound is described. The assay exhibited a good analytical recovery (>95%), precision (coefficient of variation < 10%), and linearity in plasma and whole blood specimens. The percentage cross reactivity of the major CSA metabolites M-17, M-1, and M-21 was <2%. The RIA gave values that were on average indistinguishable from those obtained by high-pressure liquid chromatography in plasma and whole blood specimens obtained from 49 renal transplant recipients.This publication has 6 references indexed in Scilit:
- Evaluation of cyclosporine kit utilizing an iodinated tracer and double antibody precipitationClinical Biochemistry, 1987
- Potential of monoclonal antibodies to improve therapeutic monitoring of cyclosporine.Clinical Chemistry, 1987
- IN VITRO IMMUNOSUPPRESSIVE PROPERTIES OF CYCLOSPORINE METABOLITESTransplantation, 1987
- IMMUNOSUPPRESSIVE METABOLITES OF CYCLOSPORINE IN THE BLOOD OF RENAL ALLOGRAFT RECIPIENTSTransplantation, 1986
- CYCLOSPORIN INCREASES THE RATE AND LENGTH OF REMISSIONS IN INSULIN-DEPENDENT DIABETES OF RECENT ONSETThe Lancet, 1986
- Use of 125I-labeled-histamine-cyclosporin C for monitoring serum cyclosporine concentrations in transplantation patients.Clinical Chemistry, 1986