A Method for the Determination of Carotene and Vitamin A in Human Blood Serum
Open Access
- 1 March 1959
- journal article
- research article
- Published by Elsevier in The American Journal of Clinical Nutrition
- Vol. 7 (2) , 197-202
- https://doi.org/10.1093/ajcn/7.2.197
Abstract
A method for determining total carotene and vitamin A in human serum has been described in detail. The usual procedure of protein precipitation with ethanol, saponification and petroleum ether extraction was followed. Total carotene was measured by a transmission reading at 452 mµ. Vitamin A was determined according to the Carr-Price method with transmission reading at 620 mµ and correction for carotene interference. Solvents, standards, reagents, and apparatus used have been described because they may greatly influence the results. The calibration graphs and details of the method for calculating the results have been given.Keywords
This publication has 1 reference indexed in Scilit:
- THE DETERMINATION OF VITAMIN A AND CAROTENE IN SMALL QUANTITIES OF BLOOD SERUMJournal of Biological Chemistry, 1946