An In Vitro Model of the Anamnestic Response of Rabbit Lymph Node Cell Suspensions to Keyhole Limpet Hemocyanin

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Abstract
Suspensions of popliteal lymph node cells from rabbits primed with keyhole limpet hemocyanin (KLH) were stimulated in vitro with KLH to give an anamnestic response. Newly synthesized IgG antibody as determined by the incorporation of radioactive leucine was first detected in the culture medium on days 2 and 3, reached a peak on days 5 to 7 and declined thereafter. The antigen-stimulated cells also synthesized increased amounts of non-antibody proteins, including α, β and γ globulins, which accounted for over 90% of the incorporated radioactivity. Both antibody and protein syntheses were antigen dose-dependent and required contact of primed cells with KLH for several hours. The synthesis of DNA and RNA increased in antigen-stimulated cells and preceded the appearance of radioactive antibody.

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