SIMPLE METHOD OF PRODUCING A MONOLAYER OF CERVICAL CELLS FOR DIGITAL IMAGE-PROCESSING

  • 1 January 1979
    • journal article
    • research article
    • Vol. 1  (2) , 84-88
Abstract
A manual method of producing a monolayer of isolated and evenly distributed [human] cervical epithelial cells was developed for use in an automated digital image analysis system [to screen for cervical cancer]. The cervical scrape specimen is vortexed from the spatula into a solution of Mucosol. The suspension is filtered through a 10 .mu. nylon mesh to remove polys and debris and then syringed to disaggregate epithelial clumps. The cells are plated out on a Nuclepore filter. The filter is touched directly onto a glass microscopic slide to transfer cells, which are then fixed and stained in a routine manner. Cell counts reveal elimination of 92% polys, with only 2% loss of squamous cells.

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