THE INCORPORATION OF TRITIATED THYMIDINE INTO A MICROSOMAL FRACTION FROM HELA CELLS DURING SHORT TIME EXPOSURES
Open Access
- 1 June 1962
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 48 (6) , 1031-1035
- https://doi.org/10.1073/pnas.48.6.1031
Abstract
When high concentrations of deoxyribonucleic acid (DNA) were included in the homogenization medium, it was possible to isolate a microsomal fraction from HeLa cells which contained a major part of the newly synthesized DNA of the cells. Kinetic studies with very short exposure times suggested that dTR-H3 incorporation into this fraction actually preceded any incorporation into the nuclei. An examination of the distribution of DNA polymerase activity in the cells showed that 90% of this enzyme was in the soluble fraction, 10% on the microsomes, and essentially none in the nuclei.Keywords
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