Mechanisms of white cell reduction in red cell concentrates by filtration: the effect of the cellular composition of the red cell concentrates
- 1 January 1993
- journal article
- Published by Wiley in Transfusion
- Vol. 33 (1) , 42-50
- https://doi.org/10.1046/j.1537-2995.1993.33193142309.x
Abstract
The effect of platelets on the removal of white cells (WBCs) from 16 to 24‐hour‐old red cell (RBC) concentrates by filtration was studied. RBC concentrates with various concentrations of platelets and WBCs were filtered on a cellulose acetate column filter and on three polyester flatbed filters. The microscopic study revealed that lymphocytes and most monocytes were captured in the smaller pores of the fiber network, irrespective of the brand of filter, the type of filter material, or the prefiltration platelet amount in the RBC concentrates. In contrast, efficient granulocyte depletion depended on granulocyte‐platelet interaction and on the filter material. In the presence of platelets, granulocytes were captured in the top part of the column filter or in the coarse layers of two of the flatbed filters, where platelets covered the fibers. Platelet depletion of the RBC concentrates prior to filtration diminished the contribution of these parts of the filters to granulocyte capture. A larger part of the column filter or the fine layers of the flatbed filters were now required for granulocyte capture. In one of the flatbed filters, granulocyte‐platelet interaction occurred mainly in the fine layers, which ended in blockage of this filter after the filtration of variable volumes (250‐600 mL) of standard RBC concentrates. A quantitative estimation of the effect of platelets on the WBC‐reduction capacity found that all three flatbed filters had a highly significant decrease (p = 0.001) in WBC‐reduction capacity for platelet‐depleted or buffy coat‐depleted RBC concentrates, as compared with standard RBC concentrates.(ABSTRACT TRUNCATED AT 250 WORDS)Keywords
This publication has 32 references indexed in Scilit:
- PADGEM-dependent adhesion of platelets to monocytes and neutrophils is mediated by a lineage-specific carbohydrate, LNF III (CD15)Cell, 1990
- White cell counting in red cells and platelets: how few can we count?Transfusion, 1990
- Use of the Sepacell filter for preparing white cell‐depleted red cellsTransfusion, 1988
- Adherence of granulocytes to nylon fibers. Evidence for a plasma granulocyte adherence factorThrombosis Research, 1988
- Herstellung von BlutkomponentenPublished by Springer Nature ,1988
- Leukocyte depletion of red cell units at the bedside by transfusion through a new filterTransfusion, 1987
- The IIb-IIIa glycoprotein complex that mediates platelet aggregation is directly implicated in leukocyte adhesionCell, 1986
- The manual preparation of leukocyte‐poor red cells for transfusion by a new filterTransfusion, 1985
- Leukocyte‐depleted blood A comparison of filtration techniquesTransfusion, 1983
- Studies on the ultrastructure of blood cells and the microaggregate fraction in stored human bloodIntensive Care Medicine, 1981