Hydrogen peroxide causes greater oxidation in cellular RNA than in DNA
- 1 January 2005
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry
- Vol. 386 (4) , 333-7
- https://doi.org/10.1515/bc.2005.040
Abstract
Human A549 lung epithelial cells were challenged with 18O-labeled hydrogen peroxide ([18O]-H2O2), the total RNA and DNA extracted in parallel, and analyzed for 18O-labeled 8-oxo-7,8-dihydroguanosine ([18O]-8-oxoGuo) and 8-oxo-7,8-dihydro-2'-deoxyguanosine ([18O]-8-oxodGuo) respectively, using high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-MS/MS). [18O]-H2O2 exposure resulted in dose-response formation of both [18O]-8-oxoGuo and [18O]-8-oxodGuo and 18O-labeling of guanine in RNA was 14-25 times more common than in DNA. Kinetics of formation and subsequent removal of oxidized nucleic acids adducts were also monitored up to 24 h. The A549 showed slow turnover rates of adducts in RNA and DNA giving half-lives of approximately 12.5 h for [18O]-8-oxoGuo in RNA and 20.7 h for [18O]-8-oxodGuo in DNA, respectively.Keywords
This publication has 33 references indexed in Scilit:
- The transcriptosomal response of human A549 lung cells to a hydrogen peroxide-generating system: relationship to DNA damage, cell cycle arrest, and caspase activationFree Radical Biology & Medicine, 2004
- Binding Capacity of Human YB-1 Protein for RNA Containing 8-OxoguanineBiochemistry, 2002
- Oxidation of 2′-deoxyguanosine by H2O2–ascorbate: evidence against free OH˙ and thermodynamic support for two-electron reduction of H2O2Journal of the Chemical Society, Perkin Transactions 2, 2001
- Singlet Oxygen Induces Oxidation of Cellular DNAJournal of Biological Chemistry, 2000
- Synthesis of a Naphthalene Endoperoxide as a Source of 18O-labeled Singlet Oxygen for Mechanistic StudiesJournal of the American Chemical Society, 2000
- In vitro Photooxidation of Nucleic Acids by Ultraviolet A RadiationPhotochemistry and Photobiology, 1997
- How Easily Oxidizable Is DNA? One-Electron Reduction Potentials of Adenosine and Guanosine Radicals in Aqueous SolutionJournal of the American Chemical Society, 1997
- FERRYL ION, A COMPOUND OF TETRAVALENT IRONJournal of the American Chemical Society, 1932
- Ueber Peroxydbildung beim EisenEuropean Journal of Organic Chemistry, 1902
- Fortsetzung der Beiträge zur nähern Kenntniss des SauerstoffsJournal für Praktische Chemie, 1860