ACTION SPECTRA FOR HEMATOPORPHYRIN DERIVATIVE AND PHOTOFRIN II WITH RESPECT TO SENSITIZATION OF HUMAN CELLSIN VITROTO PHOTOINACTIVATION

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Abstract
—Human cells of the line NHIK 3025 were labelled with hematoporphyrin derivative (Hpd) or Photofrin II (PII). Absorption and fluorescence excitation spectra of cell‐bound porphyrins were recorded. Furthermore, the corresponding action spectra for photoinactivation of the cells were determined in the spectral region 350–650 nm. The cell‐bound porphyrins were also analysed by means of HPLC. The following conclusions are drawn:(a) Porphyrins with low polarity are selectively bound to the cells.(b) Aggregated as well as unaggregated porphyrins are bound to the cells.(c) The action spectra indicate that the aggregated porphyrins do not contribute significantly to the photosensitivity of the cells.(d) The action spectra closely resemble the fluorescence excitation spectra of porphyrins bound to proteins.