EFFECT OF HOMOLOGOUS DELAYED-HYPERSENSITIVITY TESTING ON SPECIFIC AND NON-SPECIFIC LYMPHOBLASTIC TRANSFORMATION IN CATTLE LATENTLY INFECTED WITH BOVINE-RHINOTRACHEITIS VIRUS (BOVINE-HERPESVIRUS-1, BHV-1)

  • 1 January 1984
    • journal article
    • research article
    • Vol. 15  (4) , 483-490
Abstract
Two cattle latently infected with infectious bovine rhinotracheitis virus (Bovine herpesvirus 1, BHV 1) were intradermally injected with inactivated BHV 1 antigen (delayed hypersensitivity test, DHT, skin test). Another animal, free of BHV 1 infection was similarly treated. Two latent carriers of the virus, intradermally injected phosphate-buffered saline solution were used as control. The evolution of spontaneous multiplication of lymphocytes and lymphoblastic transformation in vitro induced by 3 phytomitogens [concanavalin A, phytohemagglutinin, pokeweed mitogen] and BHV 1 antigen was followed in all animals. The delayed hypersensitivity test provoked an increase in spontaneous lymphocyte multiplication in latent carriers as well as an increase in mitogen- and antigen-induced blastogenesis on the 2nd and the 9th day following the treatment. A similar increase occurred on the 9th day in the BHV 1-free animal. Therefore, in latent carriers of BHV 1, delayed hypersensitivity testing induces an anamnestic lymphocyte reaction corresponding to the cutaneous one. This first lymphocyte activity is followed, 1 wk later, by a new reaction which occurs both in infected and uninfected animals.

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