Freezing and Viability of Tetrahymena pyriformis in Dimethylsulfoxide
- 3 April 1964
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 144 (3614) , 64-65
- https://doi.org/10.1126/science.144.3614.64
Abstract
Ciliated protozoa may be preserved at very low temperatures. Tetrahymena pyriformis, suspended in 10 percent dimethylsulfoxide, survived when frozen in two steps, first to -20 ° C, then transferring immediately to a temperature of -196 ° C. Motility and ability to reproduce were recovered after freezing by this method and subsequent storage for 3 months in a liquid nitrogen refrigerator.Keywords
This publication has 4 references indexed in Scilit:
- Prolonged Survival of Trichomonas vaginalis at −79° C.Nature, 1959
- Preservation of trypanosomes in the frozen stateTransactions of the Royal Society of Tropical Medicine and Hygiene, 1957
- The maintenance of stock strains of trichomonads by freezingEpidemiology and Infection, 1954
- Preservation ofEntamoeba Histolyticaat −79°C. in the Presence of GlycerolPathogens and Global Health, 1953