Formation and properties of S‐protein complex with S‐peptide‐containing fusion protein
- 21 February 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 339 (3) , 209-212
- https://doi.org/10.1016/0014-5793(94)80417-6
Abstract
A fusion protein (FP) comprised of the RNase A S‐peptide and human epidermal growth factor was shown to form a stable noncovalent catalytically active complex with the RNase A S‐protein at a stoichiometric ratio 1:1 with K diss = 5.0 × 10−7M. The S‐protein complex with FP exhibits the pyrimidine specificity toward substrates in both reactions catalyzed by RNase S, transesterification and hydrolysis. The fusion protein can be determined specifically and quantitatively in the presence of S‐protein by RNase activity assays. The possibility of effective purification of S‐peptide‐containing proteins by affinity chromatography on an S‐protein‐Sepharose column has been demonstrated.Keywords
This publication has 11 references indexed in Scilit:
- Ribonuclease S‐peptide as a carrier in fusion proteinsProtein Science, 1993
- Sequence modeling using semisynthetic ribonuclease S.Journal of Biological Chemistry, 1982
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Ribonuclease S-peptide. A model for molecular recognition.Journal of Biological Chemistry, 1976
- Relationship between α-helical propensity and formation of the ribonuclease-S complexJournal of Molecular Biology, 1975
- Peptide-peptide interactions on an insoluble matrixBiochemistry, 1972
- 24 Bovine Pancreatic RibonucleasePublished by Elsevier ,1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- The Three-Dimensional Structure of Ribonuclease-SJournal of Biological Chemistry, 1970
- [76a] Preparation of ribonuclease S and its component parts, S-peptide and S-proteinPublished by Elsevier ,1967