Ultrastructural Changes in the Acrosome of Human Sperm During Freezing and Thawing: A Pilot Trial

1 January 1986

journal article
research article
Published by Taylor & Francis in Archives of Andrology

Vol. 17 (2) , 145-150
https://doi.org/10.3109/01485018608990187

Abstract

As a pilot trial, a semen sample was diluted with a glucose-citrate-egg yolk diluent and frozen in 0.5 ml PVC paillettes in liquid nitrogen vapor. Motility and acrosome integrity of the sample were evaluated before and after freezing, and longevity was monitored up to 6 h postthaw. Acrosome integrity was assessed by comparing Spermac-stained thin smears with TEM. Acrosome damage was found to be progressive, and four main types of acrosome state were identified and illustrated.

Keywords

This publication has 3 references indexed in Scilit:

Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa
Veterinary Record, 1975
Semen quality from the bull to the freezer: An assessment
Theriogenology, 1975
Ultrastructural Changes in the Human Spermatozoon after Freezing for Artificial Insemination
Fertility and Sterility, 1971