METHOD OF ESTIMATING THE BACTERIAL POPULATION ON SURFACES

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Abstract
A new method of removing the bacterial population from surfaces has been developed for use in laboratory and field studies. The method is based on the impingement of a spray of washing solution against a circumscribed area of surface; the number of bacteria in the resultant suspension is subsequently determined by plating. The equipment developed for this procedure is simple to use, portable, and does not injure the test surface. In comparative tests using chicken skin and slices of lean beef and pork fat, inoculated uniformly with Pseuiomonas fluorescens, recoveries obtained with the spray technique were superior to those obtained with conventional methods.

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