The Immunizing Effect of Calcium Phosphate Adsorbed Influenza Virus

Publisher Website
Google Scholar
Abstract
A group of Swiss mice was given individually subcut. injns. of 0.5 ml. of formalinized allantoic fluid suspension infected with PR8 strain of influenza virus, Type A; a 2d group was given similarly treated virus suspension adsorbed on Ca phosphate; a 3d group contained untreated controls. At 4. 8 and 14 wks. after vaccination, the mice were tested for immunity to intranasal infection with graded doses of mouse-adapted PR8 virus. The immunizing effect was enhanced and prolonged by the adsorption of virus on Ca phosphate. Serum antibody titers obtained from 5 mice in each group, 10 wks. after vaccination, and measured by the agglutinin-inhibition reaction, were 256 and 512, in animals vaccinated with free and adsorbed virus, respectively. The adsorbed virus had greater stability of hemagglutinating capacity in tests for 4 mos. at 4[degree]C, 37[degree]C and at room temp. Reactions were untoward and similar in human subjects who were given 1 ml. subcut. injns. of either adsorbed or non-adsorbed Type A and B viruses from formalinized allantoic fluid.