Fluorescent Oligonucleotides - Versatile Tools as Probes and Primers for DNA and RNA Analysis
- 1 October 1999
- journal article
- new tools-in-synthesis
- Published by Georg Thieme Verlag KG in Synlett
- Vol. 1999 (10) , 1667-1678
- https://doi.org/10.1055/s-1999-2914
Abstract
Different strategies for the fluorescence labeling of nucleosides and oligonucleotides and techniques for their detection are discussed. Pre- and post-synthetic routes as well as automated solid phase-based syntheses and enzymatic methods are compared. Tailor-made chemical syntheses are presented that meet the requirements of a broad spectrum of applications for oligonucleotides as probes.Keywords
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