Expression of the Rickettsia prowazekii citrate synthase gene in Escherichia coli
- 1 July 1983
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 155 (1) , 412-6
- https://doi.org/10.1128/jb.155.1.412-416.1983
Abstract
Recombinant DNA techniques were used to isolate the Rickettsia prowazekii citrate synthase gene on the plasmid vector pBR322 by functional complementation of a gltA mutation of Escherichia coli K-12. Analysis of citrate synthase activity in crude extracts revealed that the enzyme expressed in E. coli retains the regulatory control mechanisms characteristic of the rickettsial enzyme.Keywords
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