Abstract
It has been suggested that the bios requirements of yeast may vary to some extent according to the nature of the nitrogen source. In view of the undoubtedly complex nature of the synthetic reactions involved in the production of proteins by yeast from simple nitrogen compounds, such a suggestion is inherently likely. Consequently it was felt that this question should be examined more closely since it is relevant to the propriety of assessing the relative nutrient values of different nitrogen sources under constant conditions of bios dosage. The experiments reported in the following paper show that certain differences in the bios requirements of yeasts are apparent when a comparison is made between ammonia and amino acids as nutrients. These differences are, however, quite small and do not affect to any appreciable extent the assessment of their relative nutrient values. No differences in the bios requirements of amino acids between themselves have been established. It has further been shown that the bios requirements of different top-fermentation brewery yeasts may vary quite markedly; in fact, out of four strains studied three different kinds of bios requirement were found.

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