Biosynthesis of Cytidine Diphosphate Diglyceride by Enzyme Preparations from Cauliflower

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Abstract
The activity of the enzyme responsible for the synthesis of cytidine diphosphate diglyceride has been found in the 20,000g pellet of a cauliflower (Brassica oleracea) inflorescence homogenate. Greater enzyme activity was found in the pellet after treatment with 0.02% Triton X-100, or after freezing and thawing. Activity was greater when fluoride was included in the reaction mixture. The enzyme has a pH optimum of 5.6, an optimal Mn2+ concentration of 1mm and an optimal Mg2+ concentration of 30 mm. At the optimal concentration, Mn2+ was 4 times better than Mg2+. The utilization of biosynthesized cytidine diphosphate diglyceride can be enhanced by addition of inositol, thereby causing the formation of phosphatidyl inositol.