Induction of CD8+ cytotoxic T lymphocytes by immunization with syngeneic irradiated HIV-1 envelope derived peptide-pulsed dendritic cells

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Abstract
Based on the evidence that CD8+; cytotoxic T cells (CTL) precursors do not appear to distinguish between virus-infected cells and viral peptide-puised syngeneic cells, we have developed methods for priming class I MHC molecule restricted CD8+; CTL with such peptides without using any adjuvant. We were able to prime in vivo such CTL immunity lasting at least 6 months with a single i.v. injection of syngeneic 2200–3300 red irradiated peptide-pulsed spleen cells, and even more efficiently with a very small number of irradiated class II MHC molecule expressing spienic dendritic cells (DC). No foreign serum source was necessary during the puising. Interestingly, we could not generate significant CTL activity with unirradiated or low dose (in vivo. These results offer useful information for development of synthetic peptide vaccines and immunotherapy.

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