Normal-phase high-performance liquid chromatographic determination and identification of aldrin, dieldrin, and DDTs in eggs.
Open Access
- 1 May 2001
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 39 (5) , 183-187
- https://doi.org/10.1093/chromsci/39.5.183
Abstract
A method for the routine monitoring of residual aldrin, dieldrin, pp′- DDT, op′-DDT, pp′-DDE, and pp′-DDD in eggs by high-performance liquid chromatography (HPLC) with a photo-diode array (PDA) detector is described. The lipids extracted from a whole egg are cleaned by a solid-phase extraction (SPE) ISOLUTE NH2 column with a diethyl ether-hexane (5:95, v/v) eluent. The HPLC separation is carried out using a normal-phase (LiChrosorb NH2) column, a heptane-hexane eluent (97:3, v/v), and a PDA detector. The average recoveries from fortified target compounds (0.1, 0.2, 0.3, and 0.4 µg/g, respectively) are ≥ 83%, with standard deviations (SDs) between 2 and 5%. The interassay variabilities and their SDs are ≤ 4.8% and ≤ 0.7%, with intra-assay variabilities of 2.1–3.3%. The limits of determination for these compounds range from 0.04 to 0.08 µg/g.Keywords
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