A nuclear magnetic resonance study of the ribotrinucleoside dlphophate UpUpC

Abstract
500 MHz 1H, 67.89 MHz 13C and 80.97 MHZ 31P-NMR studies are reported on the ribotrinucleoside diphosphate UpUpC, the triplet codon corresponding to the amino acid phenylalanine. Complete spectral assignments are given and con-formational parameters for the backbone and the furanose rings are determined. All three nucleotide units show a near-balance for the N/S equilibrium with a slight preference for the N-type ribose (∑60%) . The backbone conformation around the C3'-03' bonds show a preference for the trans domain, while the orientation aroung the C5'-05' bonds is predominantly trans.

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