HUMAN MIXED LYMPHOCYTE CULTURE USING SEPARATED LYMPHOCYTE POPULATIONS

Google Scholar
  • 1 January 1977
    • journal article
    • research article
    • Vol. 32  (3) , 359-365
Abstract
The ability of human blood lymphocyte populations enriched with T [thymus-derived] or B [bone marrow-derived] cells to act as responder and stimulator populations in the 1-way mixed lymphocyte reaction (MLR) was investigated. T- and B-cell-enriched populations were obtained by separation of rosette-forming and non-rosette-forming cells, and T-cell-enriched populations were also obtained by nylon-fiber column filtration. Using cells prepared by rosette sedimentation, control unseparated and T-cell-enriched populations responded well when stimulated by mitomycin C-treated unseparated cells from a 2nd individual; and stimulation by T- and B-enriched populations generally produced some response, although the magnitude was variable. B-cell-enriched populations gave virtually no response regardless of the composition of the stimulating populations. Nylon-column-enriched T-cell populations responded to stimulation by control unseparated cells but not to T cells purified by the same procedure. T-cell enriched populations prepared by the 2 methods thus had different activities in the MLR despite containing similar numbers of T cells. This suggests that other factors, such as the presence of small numbers of accessory cells, are important in determining the magnitude of the MLR.