Assay for trimethoprim in serum and urine by means of ion-pair chromatography.

1 December 1980

journal article
research article
Published by Oxford University Press (OUP) in Clinical Chemistry

Vol. 26 (13) , 1791-1795
https://doi.org/10.1093/clinchem/26.13.1791

Abstract

We describe a rapid, precise, and reliable procedure for assay of trimethoprim in serum and urine by ion-pair chromatography. Trimethoprim concentrations in urine are determined by an externally standardized, direct-injection procedure; assay in serum involves a simple preliminary extraction and internal standardization. The assays are suitable for pharmacokinetic studies and have been applied to determination of trimethoprim concentrations in serum and urine during therapy with Co-trimoxazole, a sulfonamide/trimethoprim preparation.

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