5-Aminolevulinic-Acid Synthetases from Rhodopseudomonas spheroides Y. Comparison of the Purification and Properties of Enzymes Extracted from Bacteria Grown in Different Iron Concentrations
- 1 November 1975
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 59 (1) , 73-77
- https://doi.org/10.1111/j.1432-1033.1975.tb02426.x
Abstract
The two 5-aminolevulinic acid synthetases of Rhodopseudomonas spheroides Y. were extracted from cells grown in a 'low-iron' medium and purified. They have a specific activity 10-fold higher than the 'high-iron' enzymes described by us previously and have the same properties except that they do not contain any iron and have one free-SH group more per mole of enzyme (2 for E1; , for E2) Their inhibition by adenosine triphosphate and iron and their oxidation-reduction sensitivity are discussed in terms of light, oxygen and heme feed-back regulation of bacteriochlorophyll sunthesis.Keywords
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