Aflatoxin M1 in Milk: Evaluation of Methods
Open Access
- 1 September 1973
- journal article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 56 (5) , 1106-1110
- https://doi.org/10.1093/jaoac/56.5.1106
Abstract
Six published methods for the determination of aflatoxin M1 in liquid and powdered milk were compared because a quantitative assay sensitive to 0.1 μg/L was needed for routine analysis. Each method was tested with both spiked and naturally contaminated samples at levels of 0.1, 0.5, and 1 μg/L or 1, 5, and 10 μg/kg; recoveries of M1 were determined. Data revealed that 2 methods, one for liquid milk and one for powdered milk, had the desired sensitivity and recoveries. In the liquid milk method, M1 is extracted and milk protein is precipitated simultaneously with methanol-water (4+1); in the powdered milk method, M1 is extracted with acetone-water (70+30) and milk protein is then precipitated with a lead acetate solution. Both methods remove fats from the aqueous acetone or methanol solutions with hexane before partition of M1 into chloroform. Aflatoxin M1 is determined by thin layer chromatography of the chloroform extracts and either visual or densitometric measurement of mycotoxin on thin layer plates. Modifications were made to simplify the methods and to reduce the time required to complete the assays.Keywords
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