Amphipathic Helix-Dependent Localization of NS5A Mediates Hepatitis C Virus RNA Replication
Open Access
- 15 May 2003
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 77 (10) , 6055-6061
- https://doi.org/10.1128/jvi.77.10.6055-6061.2003
Abstract
We identified an N-terminal amphipathic helix (AH) in one of hepatitis C virus (HCV)’s nonstructural proteins, NS5A. This AH is necessary and sufficient for membrane localization and is conserved across isolates. Genetically disrupting the AH impairs HCV replication. Moreover, an AH peptide-mimic inhibits the membrane association of NS5A in a dose-dependent manner. These results have exciting implications for the HCV life cycle and novel antiviral strategies.Keywords
This publication has 55 references indexed in Scilit:
- Hepatitis C Virus (HCV) NS5A Binds RNA-dependent RNA Polymerase (RdRP) NS5B and Modulates RNA-dependent RNA Polymerase ActivityJournal of Biological Chemistry, 2002
- A Human Hepatoma Cell Line Expressing Hepatitis C Virus Nonstructural Proteins Tightly Regulated by TetracyclineBiochemical and Biophysical Research Communications, 2001
- Hepatitis C Virus RNA Codes for Proteins and Replicates: Does It also Trigger the Interferon Response?Seminars in Liver Disease, 2000
- Efficient Initiation of HCV RNA Replication in Cell CultureScience, 2000
- The Prevalence of Hepatitis C Virus Infection in the United States, 1988 through 1994New England Journal of Medicine, 1999
- THE GREEN FLUORESCENT PROTEINAnnual Review of Biochemistry, 1998
- Characterization of the nuclear localization signal and subcellular distribution of hepatitis C virus nonstructural protein NS5AGene, 1996
- Sorting and retrieval between the endoplasmic reticulum and Golgi apparatusCurrent Opinion in Cell Biology, 1995
- Molecular and ultrastructural analysis of heavy membrane fractions associated with the replication of Kunjin virus RNAArchiv für die gesamte Virusforschung, 1992
- Association of Foot-and-Mouth Disease Virus Replicase with RNA Template and Cytoplasmic MembranesJournal of General Virology, 1974