Extraction and purification of a substance with luteinizing hormone releasing activity from the leaves of Avena sativa.

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Abstract
Attempts were made to purify the LH [luteinizing hormone]-releasing substance extracted from the leaves of A. sativa by means of 2-step chromatographic procedures using a weakly acidic ion-exchange resin and DEAE-Sephadex A-25 (coarse) with successful results. For preliminary fractionation of such starting materials as dried leaves, fresh leaves and acetone-extracted powder (crude extracts), 5% acetate-buffered active C proved to be more effective than starch zone electrophoresis. From its behavior on chromatography with weakly acidic ion-exchange resins as well as Sephadex gel filtration, the active fraction extracted from the leaves of A. sativa was assumed to be different from the LH-RH [luteinizing hormone-releasing hormone] present in the hypothalamus. This partially purified material, however, was demonstrated to have an LH-releasing activity by the ovarian ascorbic acid depletion method using Wistar-Imamichi strain rats. Its site of action is apparently in the adenohypophysis.

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