Biosynthetic Origin of Gibberellins A3 and A7 in Cell-Free Preparations from Seeds of Marah macrocarpus and Malus domestica

Open Access
Publisher Website
Google Scholar
Abstract
Cell-free preparations from seeds of Marah macrocarpus L. and Malus domestica L. catalyzed the conversion of gibberellin A9 (GA9) and 2,3-dehydroGA9 to GA7; GA9 was also metabolized to GA4 in a branch pathway. The preparation from Marah seeds also metabolized GA5 to GA3 in high yield; GA6 was a minor product and was not metabolized to GA3. Using substrates stereospecifically labeled with deuterium, it was shown that the metabolism of GA5 to GA3 and of 2,3-dehydroGA9 to GA7 occurs with the loss of the 1β-hydrogen. In cultures of Gibberella fujikuroi, mutant B1-41a, [1β,2β-2H2]GA4, was metabolized to [1,2-2H2]GA3 with the loss of the 1α- and 2α-hydrogens. These results provide further evidence that the biosynthetic origin of GA3 and GA7 in higher plants is different from that in the fungus Gibberella fujikuroi.

This publication has 15 references indexed in Scilit: