Effect of ECS and puromycin on memory in fish.

Abstract
Five hundred and seventy goldfish, trained in shuttle boxes to swim from a light to a dark compartment to avoid electrical shock, were given 20 learning trials on 1 day and 10 retention crials 3 days later. ECS within 90 min. or intracranial injection of puromycin within 30 min. after Trial 20 caused significant deficits in retention. Subconvulsive electric shock had no significant effect. Cooling Ss for 2 hr. following Trial 20 prolonged the interval during which ECS could cause a retention deficit.

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