Heat‐stable serotyping antigens expressed by strains of Campylobacter jejuni are probably capsular and not long‐chain lipopolysaccharide
- 1 December 1996
- journal article
- Published by Wiley in Journal of Applied Bacteriology
- Vol. 81 (6) , 635-640
- https://doi.org/10.1111/j.1365-2672.1996.tb03558.x
Abstract
The role of lipopolysaccharide (LPS) in the serotyping of Campylobacter jejuni based on heat-stable antigens was examined using SDS-PAGE and a silver stain for carbohydrate. None of the 32 type strains of Camp. jejuni expressed long-chain LPS. Rabbit antibodies, prepared to 10 selected strains of Camp. jejuni, reacted with surface-exposed carbohydrate antigens, which were not LPS. This study suggests that the heat-stable antigens of Camp. jejuni, which form the basis for the established Penner serotyping scheme, are probably capsular and not LPS.Keywords
This publication has 32 references indexed in Scilit:
- A rapid immunoblotting procedure for detecting serum antibodies to the lipopolysaccharide of Escherichia coli 0157Letters in Applied Microbiology, 1994
- Heterogeneity in expression of lipopolysaccharides by strains of Escherichia coli O157Journal of Infection, 1993
- Antibodies to lipopolysaccharide and outer membrane proteins ofSalmonella enteritidisPT4 are not involved in protection from experimental infectionFEMS Microbiology Letters, 1991
- Purification of lipopolysaccharide from strains ofYersinia enterocoliticabelonging to serogroups 03 and 09FEMS Microbiology Letters, 1991
- Serological identification of infection by Vero cytotoxin producing Escherichia coli in patients with haemolytic uraemic syndromeSerodiagnosis and Immunotherapy in Infectious Disease, 1990
- Conversion ofSalmonella enteritidisphage type 4 to phage type 7 involves loss of lipopolysaccharide with concomitant loss of virulenceFEMS Microbiology Letters, 1989
- Antigenic Alteration of Contaminating Lipopolysaccharide during Extraction of Escherichia coli Outer-membrane Proteins from Polyacrylamide GelsMicrobiology, 1985
- Serotyping ofCampylobacter jejuni andCampylobacter coli on the basis of thermostable antigensEuropean Journal of Clinical Microbiology & Infectious Diseases, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970