Oxidative Activity of Particles prepared from Lettuce Seedlings

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Abstract
A particulate fraction similar to mitochondria was isolated from germinating lettuce seedlings. The endogenous oxygen uptake of this fraction was very high. It could oxidize most of the Krebs cycle intermediates, the oxidation of l-malate being particularly marked. Glutamic, glycollic, and formic acids could also be oxidized to a certain extent. Fatty acids inhibited the endogenous respiration, the inhibition increasing with increased concentration. Coumarin and thiouren do not seem to have any direct effect on the respiratory enzyme systems. They apparently affect the respiration of whole seeds only indirectly, maybe through some germination regulating mechanism not yet known.

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