Discovery of Sonic hedgehog expression in postnatal growth plate chondrocytes: Differential regulation of Sonic and Indian hedgehog by retinoic acid
- 1 January 2002
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 87 (2) , 173-187
- https://doi.org/10.1002/jcb.10285
Abstract
Sonic hedgehog (Shh) is a key signal protein in early embryological patterning of limb bud development. Its analog, Indian hedgehog (Ihh), primarily expressed during early cartilage development in prehypertrophic chondrocytes, regulates proliferation and suppresses terminal differentiation of postnatal growth plate (GP) chondrocytes. We report here for the first time that both Shh and Ihh mRNA are expressed in the GP of rapidly growing 6‐week‐old broiler‐strain chickens. They are also expressed in other tissues such as articular chondrocytes, kidney, and bone. In situ hybridization and RT‐PCR analyses reveal Shh in all zones of the GP, with peak expression in late hypertrophy. Using primary cultures of GP chondrocytes in serum‐containing medium, we followed the patterns of Shh and Ihh mRNA expression as the cultures matured and mineralized. We find a cyclical expression of both hedgehog genes during the early period of culture development between day 10 and 14; when one is elevated, the other tended to be suppressed, suggesting that the two hedgehogs may play complementary roles during GP development. Retinoic acid (RA), a powerful modulator of gene expression in cell differentiation, stimulates GP chondrocytes toward terminal differentiation, enhancing mineral formation. We find that RA strongly suppresses Ihh, but enhances expression of Shh in this system. While Ihh suppresses maturation of GP chondrocytes to hypertrophy, we hypothesize that Shh acts to push these cells toward hypertrophy. J. Cell. Biochem. 87: 173–187, 2002.Keywords
This publication has 64 references indexed in Scilit:
- Direct Inhibition of Indian Hedgehog Expression by Parathyroid Hormone (PTH)/PTH-Related Peptide and Up-Regulation by Retinoic Acid in Growth Plate Chondrocyte CulturesExperimental Cell Research, 2001
- Effects of Calcitonin and Parathyroid Hormone on Calcification of Primary Cultures of Chicken Growth Plate ChondrocytesJournal of Bone and Mineral Research, 1997
- Differential regulation of type‐II and type‐X collagen synthesis by parathyroid hormone‐related protein in chick growth‐plate chondrocytesJournal of Orthopaedic Research, 1997
- Bcl-2 Lies Downstream of Parathyroid Hormone–related Peptide in a Signaling Pathway That Regulates Chondrocyte Maturation during Skeletal DevelopmentThe Journal of cell biology, 1997
- Bone growth: Coordinating chondrocyte differentiationCurrent Biology, 1996
- Uptake and metabolism of [3H]retinoic acid delivered to human foreskin keratinocytes either bound to serum albumin or added directly to the culture mediumBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1996
- Inhibition of the chondrocyte phenotype by retinoic acid involves upregulation of metalloprotease genes independent of TGF‐βJournal of Cellular Physiology, 1994
- Localization of Osteogenic Protein-1 (Bone Morphogenetic Protein-7) during Human Embryonic Development: High Affinity Binding to Basement MembranesBiochemical and Biophysical Research Communications, 1994
- Mutations affecting segment number and polarity in DrosophilaNature, 1980
- Reproduction and vision in rats maintained on a retinol-free diet containing 3-dehydroretinol (vitamin A2)British Journal of Nutrition, 1967