Application of Micro-Flow Fluorometry to Studies of Meiosis in the Male Rat

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Abstract
Suspensions of testicular cells from 15 to 150 day old rats were stained with a fluorochrome (ethidium bromide) and subjected to micro-flow fluorometry. The resulting DNA distribution patterns consisted of two to three peaks of fluorescence whose relative size altered with increasing age. Up to Day 20, two peaks of fluorescence were detected. One represented diploid cells (2C) and the other tetraploid cells (4C). Following a relative accumulation of tetraploid cells, beginning at Day 19, a third peak of fluorescence (1C) representing a haploid cell population became apparent in cell suspensions from animals 20 days of age. The size of the peak gradually increased in relative magnitude until Day 60 when the haploid cells constituted approximately 75 percent of the total testis cell population.